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brucei but not the human UBA1. This is particularly the situation for TbUBA1b, the protein with the best job in viability. Whereas the space in TbUBA1a seems restricted throughout the gatekeeper residue, this isn't the situation for TbUBA1b due to existence of Y628 (Fig. 6C). Also, the pocket of TbUBA1b lined by G630, S631, Y660, T657 is more time